Brucellosis (cattle)

Description

Bovine brucellosis is a highly contagious disease caused by Brucella abortus. Australia has been free of the disease since 1989 and now bovine brucellosis is considered to be exotic to Australia. The major clinical feature of infection is multiple late term abortions and infertility in cattle.

Diagnosis and tests available

Diagnosis

Diagnosis is strongly supported by history, clinical signs, and confirmed by positive culture and serology for Brucella spp.

Tests available

Test

Sample(s) required

Days of the week test is conducted

Turnaround time1

Brucella abortus CFT

Clotted blood (red top tube)

Monday to Friday

Up to 5 days

Brucella abortus ELISA

Clotted blood (red top tube)

Thursday and Friday

Up to 5-7 days

Brucella abortus RBT

Clotted blood (red top tube)

Monday to Friday

Up to 5 days

Brucella abortus SAT

Clotted blood (red top tube)

Wednesday and Thursday

Up to 5 days

Culture for Brucella spp.

Fresh tissue or body fluids

Monday to Saturday2

7 days

1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.
2 Prior approval of delivery and testing on Saturday is required. Please contact Customer Service on 1800 675 623 to seek approval.

Additional Information

Specimen requirements

Specimens as required for the diagnosis of abortion in cattle (see Abortion Investigation)

Blood (without anti-coagulant) - submit chilled
  • 10ml of blood (including at least 2 ml of serum) collected into a plain red top tube
Body fluids - submit chilled
  • Pericardial, thoracic or peritoneal fluid and heart blood from foetus
  • Milk, uterine discharges or vaginal mucus from cows
  • Semen from bulls
Fresh tissue - submit chilled
  • Fresh foetal stomach contents, placenta, liver, lung and spleen
  • Whole foetus and foetal membranes

Note

  • Provide clear documentation on the Veterinary specimen advice form of each animal including the animal that aborted.
  • Blood samples collected in vacutainer containing separation gel are not suitable for Complement Fixation Test (CFT) or Rose Bengal Test (RBT). Plain tubes (red top) are recommended for blood collection.
  • Fees for tests undertaken to confirm or exclude a diagnosis of bovine brucellosis are paid by NSW Department of Primary Industries

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPI's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS

Phone1800 675 623
Email

laboratory.services@dpi.nsw.gov.au

Operating Hours

8:30am-4:30pm Monday-Friday (excluding public holidays)

8:30am-12:00pm Saturday (for deliveries only)

Postal Address

Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

For Edible oil & oilseed or Feed quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wagga Wagga on (02) 6938 1957

For Plant nutrition, Soil health or Water quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wollongbar on (02) 6626 1103

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpi.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the SVDL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type

Laboratory

Street Address

Animal (including aquatic animals)

NSW Animal and Plant Health Laboratories (APHL)

EMAI, Woodbridge Road, Menangle NSW 2568