Ref Code: SHE02

Category    

Teaching  - 3
(Instructor : student ratio 1:3)
Research - 5

Objective

To collect blood into a sterile vacutainer.

Alternatives to animal use for teaching

None practical

Details of procedure

The sheep can either be restrained in a sitting or standing position. Wool is clipped from the area of the jugular vein and disinfected with a swab of antiseptic. To take blood, the catcher places the web and extended thumb and forefinger over the thoracic inlet and squeezes. Both jugulars fill and are extremely easy to puncture to collect blood. If the needle is slightly inaccurately placed, a small amount of movement will generally rectify this very quickly.

Blood vessels on the non-wool area of sheeps’ legs can also be used for blood collection.

Drugs, chemicals or biological agents

Antiseptic for venipuncture site.

Impact of procedure on wellbeing of animal(s)

Low impact, unless excessive volumes of blood are taken. Damage to jugular can cause haematoma.

Reuse and repeated use

One animal for each attempt at collecting blood.

Care of animals during/after the procedure

This does not usually cause trauma to the sheep, so in most cases there is no need for special care after the procedure.

Pain relief measures

Not usually needed.

Qualifications, experience or training necessary to perform this procedure

Operators should be appropriately trained as skill is needed to locate the jugular and insert the needle accurately.