Genetic testing

Description

The Biotechnology laboratory at NSW DPI Laboratory Services offers a range of DNA extraction and genotyping services to identify inherited conditions in livestock. Testing is conducted Monday to Friday.

Tests available

Test

Sample(s) required

TAT1

DNA extraction

Contact lab for suitability

10D

A2 milk protein

30-40 Tail hairs, ear notch

10D

alpha-Mannosidosis  (Angus, Murray Greys or Galloways)

30-40 Tail hairs, ear notch

10D

Brachygnathia, Cardiomegaly and Renal Hypoplasia Syndrome

Ear notch (Allflex TSU)

10D

Bovine Leucocyte Adhesion Deficiency

30-40 Tail hairs, ear notch

10D

beta-Mannosidosis (Salers)

30-40 Tail hairs, ear notch

10D

Claudin 16 (Wagyu )2

30-40 Tail hairs, ear notch

10D

Coat Colour Dominant Black Allele2

30-40 Tail hairs, ear notch

10D

Coat Colour Recessive Red Allele

30-40 Tail hairs, ear notch

10D

Chediak Higachi Syndrome (Wagyu)2

30-40 Tail hairs, ear notch

10D

Citrullinaemia

30-40 Tail hairs, ear notch

10D

Congenital Myasthenic Syndrome (Brahmans)

30-40 Tail hairs, ear notch

10D

Ovine dermatosparaxis (White Dorper sheep)

Ear notch (Allflex TSU)

10D

Deficiency of Uridine Monophosphate Synthase

30-40 Tail hairs, ear notch

10D

Pompes Disease - E7, E13 or E18 mutations  (Brahmans or Shorthorns)

30-40 Tail hairs, ear notch

10D

Factor XI or Factor XIII Deficiency (Wagyu)2

30-40 Tail hairs, ear notch

10D

Factor XI Deficiency (Holstein Friesians )

30-40 Tail hairs, ear notch

10D

Freemartinism (Male-Specific PCR)3

EDTA blood 3

10D

Hyperkalemic Periodic Paralysis (Quarter Horses)

30-40 Tail hairs, ear notch

10D

Inherited congenital myoclonus

30-40 Tail hairs, ear notch

10D

Lavender Foal Syndrome

30-40 Tail hairs, ear notch

10D

Milk Protein beta-Lactoglobulin

30-40 Tail hairs, ear notch

10D

Milk Protein kappa-Casein

30-40 Tail hairs, ear notch

10D

Male-Specific PCR (Freemartinism)3

EDTA blood3

10D

Maple Syrup Urine Disease (Poll Herefords or Poll Shorthorns)

30-40 Tail hairs, ear notch

10D

Myophosphorylase Deficiency

30-40 Tail hairs, ear notch

10D

Myostatin Gene C313Y (Double-Muscling)

30-40 Tail hairs, ear notch

10D

Myostatin Gene nt821 del11 (Double-Muscling)

30-40 Tail hairs, ear notch

10D

Niemann Pick Type C

30-40 Tail hairs, ear notch

10D
Perinatal Weak Calf Syndrome in Wagyu cattle

30-40 Tail hairs, ear notch

10D

Protoporphyria

30-40 Tail hairs, ear notch

10D

Pulmonary Hypoplasia with Anasarca in Persian sheep

Ear notch (Allflex TSU)

10D

Spherocytosis (Wagyu)2

30-40 Tail hairs, ear notch

10D

   

Turnaround times are provided as a guide only (D=working days). For specific information about your submission please contact Customer Service.

2 This test is not NATA accredited.

DNA extraction fee applies

Specimen requirements

Blood (with anti-coagulant)

  • 10 ml of blood collected into an EDTA (purple top tube)
    • Submit chilled
  • A DNA extraction surcharge applies for blood samples

Fresh tissue

  • Ear notch samples are preferred. To enquire about suitability of alternative tissue samples contact Customer Service.
  • Only the Afflex© Tissue Sampling Unit (TSU) is accepted without additional charge.
    • Submit chilled

Hair

  • 30-40 tail hairs (including the bulb) from each animal. Submit in paper envelopes.
  • For more information refer to the sample collection guide

Other

  • DNA can normally be extracted from most nucleated cells however; a DNA extraction charge may apply for non-routine samples.  To enquire about suitability of alternative sample types (e.g. semen) contact Customer Service.

Additional information

A2 Genotyping

NSW Department of Primary Industries is an accredited and registered A2 Gene Tester for the A2 Corporation Ltd. Your name, contact details and information relating to the A2 testing that is to be carried out, will be disclosed to the A2 Corporation Ltd for the purpose of communicating with you and in order to maintain a register of A2 gene tested animals. Information will be stored and managed in accordance with provisions under the Privacy and Personal Information Protection Act 1998. The supply of your details is voluntary; however, the Department is unable to process your samples if the information is not provided.

You may access or correct your personal information by contacting Customer Service on 1800 675 623  or by emailing laboratory.services@dpi.nsw.gov.au.

International submissions

In addition to the NSW DPI Laboratory Services specimen advice, international submissions must also be accompanied by:

  1. a copy of a valid import permit.
  2. a completed and signed copy of the manufacturer’s declaration.

Both accompanying documents should be affixed to the outside of the package.

Suspect inherited conditions

NSW DPI Laboratory Services can investigate suspect inherited genetic conditions upon request. For such investigations you will be required to:

  • collect property and disease history
  • describe clinic presentation (photos and videos are recommended)
  • obtain pedigree information of affected animal
  • collect two blood (with anti-coagulant; EDTA) samples from affected individual and both parents
  • collect tissue (spleen or liver preferred) for affected animal. Collection vials can be provided by the laboratory with advance notice (i.e. planned euthanasia)
  • collect 30-40 tail hairs from affected animal and both parents.