Histopathology

Description

The histology laboratory prepares slides for microscopic examination which supports the team of dedicated veterinary pathologists to observe and interpret morphological changes in animal tissue. Tissue specimens can be collected from post mortems (field or in-house) or biopsies from live animals.

Terrestrial and aquatic histopathology is available in addition to unusual species on request (eg. snail, bee, tick).

The process of producing a slide for microscopic examination involves sample processing, embedding, sectioning, staining and cover slipping. Slides are then digitally scanned for interpretation by the veterinary pathologist.

Tests available

Test

Sample(s) required

Days of the week test is conducted

Turnaround time1

Histopathology (general tissues) + routine H&E staining of slide

Fixed tissues

Monday - Friday

Up to 5 days

Histopathology (TSE exclusion) + routine H&E staining of slide

Fixed tissues

Monday - Friday

Up to 10 days

Histopathology processing + routine H&E staining of slide + digital scanning

Fixed tissues + external hard drive/ USB

Monday – Friday

Up to 5 days

Digital scanning only

Submission of glass slides + external hard drive/ USB

Monday – Friday

Up to 2 days

Special stains

On request

Monday – Friday

Up to 5 days

1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.

Specimen requirements

Fixed tissue (general tissues)

  • Fresh tissues immediately placed in formalin
  • Tissue pieces should not be greater than 1cm cubed to ensure proper fixation
    • Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue

Fixed tissue (TSE exclusion)

  • Submission of whole brain is required to ensure preservation of TSE target sites
    • submit fixed in neutral buffered formalin at a ratio of 10:1 formalin: tissue
  • Brains submitted for TSE exclusion are processed once a week, separate to general tissues to ensure consistent and reliable results. Urgent cases can be processed out of cycle if clearly marked on the Submission Form however this may reduce the quality of the brain histology results, so should only be requested in extremely urgent cases.

Fresh tissue

  • Fresh tissues can be submitted following necropsy
  • The quality of the tissues will be reduced due to the effects of autolysis so ideally fixed tissue should be submitted in neutral buffered formalin at a ratio of 10:1 formalin: tissue

Digital histopathology

  • This service can be requested following processing of fixed tissues and routine H&E staining or on submitted glass slides
    • Please provide storage device (eg. USB) for return of digitised images

Special stains

  • A variety of special stains are maintained and can be performed on request