Infectious bovine rhinotracheitis

Description

Infectious bovine rhinotracheitis is a highly contagious respiratory disease, most commonly in feedlot cattle and affecting both young and older cattle. The viral infection alone causes only mild to moderate respiratory signs, but it predisposes to secondary bacterial pneumonia which can cause death.

Other syndromes observed include infectious pustular vulvovaginitis, balanoposthitis, conjunctivitis and encephalomyelitis (very rare).

Diagnosis and tests available

Diagnosis

Diagnosis is strongly supported by history, clinical signs, histopathology and serology tests and confirmed by PCR.

Tests available

Test	Sample(s) required	Days of the week test is conducted	Turnaround time¹
Histopathology	Fixed tissue	Monday – Friday	Up to 5D
Infectious bovine rhinotracheitis antibody ELISA	Clotted blood (red top tube)	Twice weekly	3-5D
Infectious bovine rhinotracheitis Real–time PCR	Fresh tissue, swab in PBGS and semen	According to demand	2-3D
Infectious bovine rhinotracheitis VNT	Clotted blood (red top tube)	Batch tested on Thursday	1-2W

Please Note: D= Working days, W= Weeks. This does not include Public Holidays.

¹Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.

Additional Information

Specimen requirements

Blood (without anti-coagulant)
10 ml of blood collected into a plain red top tube Submit chilled
Fixed tissue
Lung collected at post mortem Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue
Fresh tissue
Fresh lung collected at post mortem Submit chilled
Swab
Nasal or genital swab from acutely affected animals Send in viral transport media (PBGS)

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

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CONTACT DETAILS
Phone	1800 675 623
Email	laboratory.services@dpi.nsw.gov.au
Operating Hours	8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only)
Postal Address	Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

For Edible oil & oilseed or Feed quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wagga Wagga on (02) 6938 1957

For Plant nutrition, Soil health or Water quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wollongbar on (02) 6626 1103

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpi.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the SVDL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type	Laboratory	Street Address
Animal (including aquatic animals)	NSW Animal and Plant Health Laboratories (APHL)	EMAI, Woodbridge Road, Menangle NSW 2568