Akabane virus is a member of the Simbu serogroup of the family Bunyaviridae, genus Orthobunyavirus. It is spread by biting midges (Culicoides spp). Akabane virus infection is associated with abortion, stillbirth and congenital abnormalities such as arthrogryposis and hydranencephaly however, the severity of clinical signs and pathology are variable depending on the species of animal and time of infection. Disease due to Akabane virus is mostly observed in cattle due to the long period of gestation and breeding throughout the year.
Diagnosis and tests available
Diagnosis is supported by the history, clinical signs, pathology and supportive tests.
Days of the week test is conducted
Akabane virus antibody ELISA
Clotted blood (red top tube) or foetal fluid
Batched tested weekly
Up to 1 week
Akabane virus PCR
According to demand
2-3 working days
|Akabane VNT2||Clotted blood (red top tube)||Batch tested on Thursday||1-2 weeks|
Monday – Friday
Up to 2 weeks for brain samples. Up to 5 days for other sample types.
1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.
2 This test is predominantly used for regulatory testing.
Blood (without anti-coagulant)
- 10 ml of blood (including at least 2 ml of serum) from the dam or neonate that has not suckled collected in a plain red top tube.
- Submit chilled
- Do not use a serum separator tube.
- Brain, spinal cord, muscle and other tissues as indicated by gross examination
- Submit fixed in neutral buffered formalin at a ratio of 10:1 formalin: tissue
- 2 ml; preferably pericardial or pleural rather than heart blood or peritoneal fluid which are more likely to be contaminated
- Fresh brain submitted from the aborted foetus
- Appropriate tissues and fluids for serology should also be submitted to exclude Pestivirus infection which can cause arthrogryposis and CNS lesions.
- Serum samples are only useful in eliminating a diagnosis of Akabane virus infection in the dam however, the presence of antibody in an unsuckled calf or lamb is diagnostic
- Akabane virus may be detected in the brain of clinically affected new-bon calves or lambs by PCR. A negative result on this test does not preclude a diagnosis of Akabane disease as the infection has usually occurred months earlier. However, Akabane virus is more readily detected in the brains of aborted fetuses as abortion may occur relatively soon after infection.