Chalkbrood is a fungal disease of honey bee brood that infects the gut of the larvae. It is caused by a spore-forming fungus Ascosphaera apis that is consumed along with larval food. Although it can affect workers, drones, or queens it most often occurs in workers and drones. It does not infect humans and has no effect on the quality of the honey or other apiary products.
Diagnosis is strongly supported by hive inspection and is confirmed by physical examination of the affected comb. Culture is only performed when physical examination is inconclusive. PCR confirmation is available if requested.
Days of the week test is conducted
Ascosphaera apis PCR3
PCR performed from culture
Monday – Friday
Frass or fresh honeycomb
Monday – Saturday2
|Hive examination||Frass, fresh honeycomb, larvae or bees|
Monday – Friday
1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.
2 Prior approval of delivery and testing on Saturday is required. Please contact Customer Service on 1800 675 623 to seek approval.
3 This test is not NATA accredited.
|Larvae or bees|
Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.
NSW DPI's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.
|Phone||1800 675 623|
8:30am-4:30pm Monday-Friday (excluding public holidays)
8:30am-12:00pm Saturday (for deliveries only)
Private Bag 4008, Narellan NSW 2567
For Animal export, Plant health or Veterinary quotations, please complete the online quotation request
For Edible oil & oilseed or Feed quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wagga Wagga on (02) 6938 1957
For Plant nutrition, Soil health or Water quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wollongbar on (02) 6626 1103
For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.
For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.
Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the SVDL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.
Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).
Animal (including aquatic animals)
|NSW Animal and Plant Health Laboratories (APHL)|
EMAI, Woodbridge Road, Menangle NSW 2568