Pig Enteric Disease

Description

Lawsonia intracellularis (proliferative enteropathy), Brachyspira hyodysenteriae (swine dysentery) and Brachyspira pilosicoli (porcine intestinal/colonic spirochaetosis) are among the most common and economically important pathogenic bacteria responsible for diarrhoea in pigs.

Lawsonia intracellularis

Lawsonia intracellularis infection is known to be a significant cause of proliferative enteropathy (PE) in pigs. PE is characterised by diarrhoea, necrosis, haemorrhage or any combination referred commonly as “ileitis” affecting the health and production efficacy within the pig industry worldwide. Ileitis in pigs can present as both sub-clinical ileitis and clinical ileitis. Clinical ileitis can range from acute proliferative haemorrhagic enteropathy (PHE) and death in naive adult pigs to diarrhoea and wasting in growing pigs. Sub-clinical ileitis can present as impacted growth rate in the absence of diarrhoea and other clinical symptoms. L. intracellularis can be detected in both small and large intestinal samples.

Brachyspira hyodysenteriae

Brachyspira hyodysenteriae infection commonly associated with “swine dysentery” causes clinical disease characterised by mucohaemorrhagic diarrhoea, reduced growth performance and marked inflammation limited to the large intestine (caecum and/or colon).B. hyodysenteriae is frequently seen in grower / post weaning pigs and causes major losses to the industry.

Brachyspira pilosicoli

Brachyspira pilosicoli infection, linked to intestinal spirochaetosis, frequently colonises the large intestine and causes colitis, diarrhoea, reduced productivity and poor growth rate. B. pilosicoli occurs commonly in pigs at weaning and can also cause avian intestinal spirochaetosis in adult chickens.

Diagnosis and tests available

Diagnosis

Diagnosis is based on histopathology and PCR detection of enteric pathogens.

Tests available

Test1

Sample(s) required

Days of the week test is conducted

Turnaround time2

Brachyspira hyodysenteriae conventional PCR1

Faeces, intestinal contents, intestinal tissue, PCR from culture

Monday – Friday

Up to 5 days

Brachyspira pilosicoli conventional PCR1Faeces, intestinal contents, intestinal tissue, PCR from cultureMonday - FridayUp to 5 days
Lawsonia intracellularis  qPCR31

Faeces, intestinal contents, intestinal tissue

Monday - FridayUp to 5 days

Lawsonia intracellularis conventional PCR1

Faeces, intestinal contents, intestinal tissue

Monday - Friday

Up to 5 days

Pig Enteric Multiplex PCR Assay (Includes B hyodysenteriae; B pilosicoli; L intracellularis)1Faeces, intestinal contents, intestinal tissue, culture

Monday - Friday

Up to 5 days

1 This test is not NATA accredited
2 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Services on 1800 675 623 (option 1).
3 Detectable levels of Lawsonia intracellularis are reported as "capable of causing sub-clinical ileitis" or "capable of causing clinical ileitis".

Additional Information

Specimen requirements

Faeces
  • Faecal samples can be submitted from live animals
    • Submit chilled
Intestinal samples
  • Intestinal contents or a portion of small or large intestine
    • Submit chilled

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPI's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS

Phone1800 675 623
Email

laboratory.services@dpi.nsw.gov.au

Operating Hours

8:30am-4:30pm Monday-Friday (excluding public holidays)

8:30am-12:00pm Saturday (for deliveries only)

Postal Address

Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

For Edible oil & oilseed or Feed quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wagga Wagga on (02) 6938 1957

For Plant nutrition, Soil health or Water quality quotations and pricing enquiries, please contact DPI AgEnviro Labs at Wollongbar on (02) 6626 1103

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpi.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the SVDL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type

Laboratory

Street Address

Animal (including aquatic animals)

NSW Animal and Plant Health Laboratories (APHL)

EMAI, Woodbridge Road, Menangle NSW 2568