Histopathology - special stains

Description

The histopathology laboratory at SVDL can perform special stains to enhance and assist with diagnosis. Special stains are usually performed after initial examination by Haematoxylin and Eosin stain. Special stains are performed from a repeat cut section on a paraffin block.

Tests available

Test

Sample(s) required

Days of the week test is conducted

Turnaround time1

Alcian blue (mast cells)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Giemsa (micro-organisms)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Gomori’s Methenamine Silver (fungi, basement membrane)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Gram (gram negative and positive bacteria)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Luxol Fast Blue (myelin and nerve cells)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Masson’s Trichrome (muscle, fibrin,  connective tissue)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Modified Ziehl-Neelsen (chlamydia)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Periodic Acid Schiff’s (Fungi, carbohydrate, glycogen)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Perle’s Prussian Blue (hemosiderin)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Rhodanine (copper, bile)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Toluidine Blue (mast cells)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Von Kossa (calcium)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days working days

Warthin Starry (spirochetes, bacteria)

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

Ziehl-Neelsen

Fixed tissue, prepared cassette in formalin or paraffin block

Monday - Friday

2-3 working days

1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.

The special stains offered by this laboratory include, but are not limited to, those in the table above. For specific information about your submission please contact Customer Service.

Specimen requirements

Fixed tissue

  • Fresh tissues immediately placed in formalin
  • Tissue pieces should not be greater than 1cm cubed to ensure proper fixation
    • Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue

Paraffin block

  • Preprocessed paraffin block ready for sectioning

Prepared cassette in formalin

  • 5mm cut sample placed into histology cassette in 10% neutral buffered formalin for processing by laboratory