Salmonellosis

Description

Salmonellosis is caused by many serotypes of Salmonella enterica enterica which are typically host specific. For examples Salmonella enterica serovar dublin (S. dublin) and Salmonella enterica serovar typhimurium (S. typhimurium) cause infection in cattle and sheep. Transmission is generally by the oral route. The bacteria colonize the gut, penetrate the intestinal wall and multiply in the cells of the monocyte-macrophage line in the spleen and liver. Salmonella then re-enter the gut from where they are shed.

Diagnosis and tests available

Diagnosis

Diagnosis is suggested by clinical signs, histopathology and necropsy findings and confirmed by bacterial culture.

Serum samples from cattle or sheep may be tested by the serum agglutination test (SAT) for Salmonella dublin or Salmonella typhimurium.

Tests available

Test

Sample(s) required

Days of the week test is conducted

Turnaround time1

Histopathology

Fixed tissue

Monday – Friday

Up to 5 days

Selective enrichment culture for Salmonella

Fresh tissue or faeces

Monday – Saturday2

7 days

Salmonella SAT

(Salmonella dublin, Salmonella typhimurium)

Clotted blood (red top tube)

Wednesday & Thursday

Up to 5 days

1 Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.
2 Prior approval of delivery and testing on Saturday is required. Please contact the Customer Service Unit on 1800 675 623 to seek approval.

Specimen requirements

Blood (without anti-coagulant)

  • 10 ml of blood collected from live animals into a plain red top tube
    • Submit chilled

Faeces

  • Faecal samples can be submitted from live animals
    • Submit chilled

Fixed tissue

  • Sections of liver, kidney, lung, spleen, mesenteric lymph nodes, small and large intestine.
    • Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin:tissue

Fresh tissue

  • Portions of liver, kidney, lung, bile, spleen, mesenteric lymph nodes and small intestinal contents collected from dead animals
    • Submit chilled