Salmonellosis

Description

Salmonellosis is caused by many serotypes of Salmonella enterica subsp. enterica which are typically host specific. For example Salmonella enterica serovar Dublin (Salmonella Dublin) and Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) cause infection in cattle and sheep. Transmission is generally by the oral route. The bacteria colonize the gut, penetrate the intestinal wall and multiply in the cells of the monocyte-macrophage line in the spleen and liver. Salmonella then re-enter the gut from where they are shed.

Diagnosis and tests available

Diagnosis

Diagnosis is suggested by clinical signs, histopathology and necropsy findings and confirmed by bacterial culture.

Serum samples from cattle or sheep may be tested by the serum agglutination test (SAT) for Salmonella Dublin or Salmonella Typhimurium.

Tests available

Test	Sample(s) required	Days of the week test is conducted	Turnaround time¹
Salmonella Group-D Antibody ELISA	Clotted blood (red top tube)	Monday to Friday	Up to 5 working days (1-2 weeks notice is required before submitting samples)
Histopathology	Fixed tissue	Monday to Friday	Up to 5 days
Selective enrichment culture for Salmonella	Fresh tissue or faeces², or environmental samples	Monday to Saturday	3 - 7 working days³
Salmonella SAT (Salmonella Dublin, Salmonella Typhimurium)	Clotted blood (red top tube)	Wednesday & Thursday	Up to 5 days

¹Turnaround times are provided as a guide only. For specific information about your submission please contact Customer Service.
² Minimum of 10g of faeces required per animal (ruminants) for export or health testing.
³4 - 7 working days for export, health testing or environmental samples

Additional Information

Specimen requirements

Blood (without anti-coagulant)
10 ml of blood collected from live animals into a plain red top tube. Submit chilled.
Faeces
Faecal samples can be submitted from live animals. Submit chilled. minimum of 10 grams required per animal (ruminants) for export of health testing.
Fixed tissue
Sections of liver, kidney, lung, spleen, mesenteric lymph nodes, small and large intestine. Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue.
Fresh tissue
Portions of liver, kidney, lung, bile, spleen, mesenteric lymph nodes and small intestinal contents collected from dead animals. Submit chilled.

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPIRD's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS
Phone	1800 675 623
Email	laboratory.services@dpird.nsw.gov.au
Operating Hours	8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only)
Postal Address	Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

For Edible oil & oilseed or Feed quality quotations and pricing enquiries, please contact DPIRD AgEnviro Labs at Wagga Wagga on (02) 6938 1957

For Plant nutrition, Soil health or Water quality quotations and pricing enquiries, please contact DPIRD AgEnviro Labs at Wollongbar on (02) 6626 1103

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type	Laboratory	Street Address
Animal (including aquatic animals)	NSW Animal and Plant Health Laboratories (APHL)	EMAI, Woodbridge Road, Menangle NSW 2568