Mycoplasma infections cause a wide range of clinical conditions, particularly among pigs, cattle, sheep, goats, and poultry. Certain mycoplasmas are part of normal mucosal flora, and can outgrow pathogenic mycoplasmas in broth culture procedures. In general, special media is required for growth of mycoplasmas. Some mycoplasmas are slow growing, taking up to three weeks to appear in primary culture or in subcultures.
Avian mycoplasmosis is a cause of respiratory disease in chickens, turkeys, quail, game birds, pigeons and other avian species; many different Mycoplasma species are potentially involved, but particularly M.gallisepticum in chickens and M.meleagridis in turkeys. Mycoplasma synoviae can cause respiratory signs as well as tenosynovitis. Mycoplasma infection damages the lining of the upper respiratory tract and thereby predisposes birds to deeper respiratory disease involving the lungs and airsacs by secondary pathogens. Strains vary greatly in their pathogenicity.
Mycoplasmas can be spread vertically in the egg, as well as horizontally by respiratory fomites. The commercial boiler chicken industry is free from mycoplasmosis, as is much of the commercial turkey industry. Vaccination with live attenuated strains of mycoplasma, to replacement layer pullets going onto multi-age layer farms is commonly practiced: vaccinated birds become carriers of the vaccine strain. There are several non-pathogenic avian mycoplasmas such as M.gallinarum and M.gallinaceum which may be isolated from cases of upper respiratory tract disease but have no causal aetiological significance.
Diagnosis is based on clinical signs, management history and mycoplasma culture; further identification and species characterisation requires 16S-ribosomal PCR.
Test | Sample(s) required | Days of the week test is conducted | Turnaround time1 |
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16sRNA sequencing | Culture | Monday - Friday | Up to 7 days |
Fresh tissue or swab | Monday – Friday | 7D | |
Fresh tissue or swab | Friday set-up | 5-6 weeks | |
Histopathology examination | Tissue (Fixed) | Monday –Friday | Up to 5 days |
Mycoplasma gallisepticum/Mycoplasma synoviae duplex PCR* | Dry or PBGS swab (lung, tracheal, nasal, cloanal), fresh lung | Monday –Friday | 5 days |
1 Turnaround times are provided as a guide only. TAT does not include weekends or Public Holidays. For specific information about your submission please contact Customer Service.
* Denotes analyses not covered by the NATA scope of accreditation.
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Also known as Porcine enzootic pneumonia
Mycoplasmal pneumonia is a common chronic disease particularly in grower finisher pigs, and increases susceptibility of pigs to secondary infection, most commonly Pasteurella multocida, but also Arcanobacter pyogenes, Haemophilus sp, Streptococcus sp, Staphylococcus sp, Klebsiella sp, and Bordetella bronchiseptica. M. hyosynoviae also causes synovitis and arthritis.
M. hyorhinis is a common inhabitant of the URT and ears (Eustachian tube), and is a common co-inhabitant of pneumonic lungs. It has no pneumonic potential and can rapidly overgrow M. hyopneumoniae cultures. It can cause a serofibrinous polyserositis, or fibrinous arthritis in suckers or weaners less than 10 weeks old.
Diagnosis is based on clinical signs, management history and mycoplasma culture and PCR.
Test | Sample(s) required | Days of the week test is conducted | Turnaround time1 |
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Fresh tissue or swab | Monday to Friday | 7D | |
Histopathology examination | Fixed tissue | Monday to Friday | Up to 5 days |
Serum (red top) or plasma (green top) | Thursday and Friday | Up to 7 days | |
Culture | Monday to Friday | 5 days |
1 Turnaround times are provided as a guide only. TAT does not include weekends or Public Holidays. For specific information about your submission please contact Customer Service.
* Denotes analyses not covered by the NATA scope of accreditation.
Blood (with anti-coagulant) |
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Mycoplasma spp bovine Group 7 is a frequent cause of polyarthritis in calves. It can also cause outbreaks of bovine mastitis and abortion. This organism is readily cultured, and is occasionally cultivable on blood agar cultures without special mycoplasmal media. Other Mycoplasma spp are associated with bovine mastitis, eye lesions, reproductive lesions, and caprine and ovine arthritis, mastitis and pneumonia.
Diagnosis is based on clinical signs, management history and mycoplasma culture and PCR.
Test | Sample(s) required | Days of the week test is conducted | Turnaround time1 |
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16sRNA sequencing | Culture | Monday - Friday | Up to 7 days |
Fresh tissue or swab | Monday – Friday | 7D | |
Histopathology examination | Fixed tissue | Monday –Friday | Up to 5 days |
Mycoplasma bovis PCR for export to New Zealand- tested in duplicate | 5 x semen straws - Submit either chilled or frozen | Monday- Friday | Up to 5 days |
Mycoplasma bovis PCR* | Semen, milk, fresh lung | Monday- Friday | 5 days |
Mycoplasma capricolum subsp. capripneumoniae qPCR* | Fresh lung tissue, pleural fluid, mediastinal lymph nodes, or swabs thereof and bronchoalveolar washings. | Monday- Friday | 5 days |
Mycoplasma ovis PCR* | EDTA, fresh spleen | Monday- Friday | 5 days |
Mycoplasma conjunctivae qPCR | Dry or PBGS Swab (Conjunctival) | Monday- Friday | 5 days |
Ovine pinkeye panel PCR- Moraxella ovis, Mycoplasma conjunctivae, Chlamydia pecorum * | Conjunctival Swab (Dry or in PBGS) | As required | 5 days |
Bovine pinkeye panel PCR- Moraxella bovis, Moraxella bovoculi, Mycoplasma bovis* | Conjunctival Swab (Dry or in PBGS) | As required | 5 days |
1 Turnaround times are provided as a guide only. TAT does not include weekends or Public Holidays. For specific information about your submission please contact Customer Service.
* Denotes analyses not covered by the NATA scope of accreditation.
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Swab |
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Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.
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CONTACT DETAILS | |
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Phone | 1800 675 623 |
Operating Hours | 8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only) |
Postal Address | Private Bag 4008, Narellan NSW 2567 |
Veterinary Specimen Advice Form
Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.
For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.
For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.
Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.
Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).
Sample Type | Laboratory | Street Address |
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Animal (including aquatic animals) | NSW Animal and Plant Health Laboratories (APHL) | EMAI, Woodbridge Road, Menangle NSW 2568 |