Bovine diarrhoea is a disease syndrome that can present for a number of different reasons. Age, environment, animal immunity, diet and husbandry are all influencing factors. There are several infectious causes that are important to exclude when investigating possible causes of diarrhoea. Colour and consistency of the faeces alone cannot be used to determine the cause of diarrhoea and diagnostic tests are indicated. Concurrent infections are possible and common.
Escherichia coli K99
Enterotoxigenic E. coli K99 is one of the more pathogenic E. coli strains that has been identified as a cause of diarrhoea in neonates. Susceptibly is age specific, with fimbria only able to adhere to the intestinal epithelium within approximately 2 days of birth. Concurrent infections can extend this time of susceptibility, with disease commonly occurring within the first 4 days of life.
Many different strains of Salmonella have been identified as a cause of diarrhoea in cattle of all ages. In addition to diarrhoea, Salmonellosis can also present as respiratory disease, joint-ill and sudden death. To determine treatment and prevention options, selective culture is necessary to enable serotyping of any isolates. Antibiotic resistance is reported in Australia, therefore, performing antibiotic sensitivity on any Salmonella isolates is recommended. Antibiotic sensitivity and Salmonella serotyping may be requested following isolation of Salmonella (additional charges apply).
Yersinia
Yersinia pseudotuberculosis and Yersinia enterocolitica are known causes of severe diarrhoea and sometimes death in cattle. Cases are most common in winter and early spring, often associated with recent flooding or waterlogged pastures.
Cryptosporidium parvum
The main species of pathogenic concern for calves is Cryptosporidium parvum. Disease is most common in calves between 4 days and 4 weeks of age.
Coccidia
Coccidiosis is typically a disease of older calves, often around weaning, however, disease can occur in calves as early as 3 to 4 weeks of age. Animals can be infected with low numbers of organism without disease. Faeces from affected calves can contain blood.
Round worms
Several species of round worms (eg Ostertagia sp., Trichostrongylus sp., Cooperia sp. and Oesophagostumum radiatum) can infect pasture-grazing cattle and be associated with diarrhoea, significantly impacting production.
Rotavirus
Rotavirus is the most commonly diagnosed cause of calf diarrhoea in Australia, commonly affecting calves between 4 days and 2 weeks of age, occasionally as old as 6 weeks old. Morbidity can be high while mortality is often low. Concurrent infections are common.
Coronavirus
A less common viral cause of diarrhoea in calves compared to Rotavirus, most often affecting calves between 1-3 weeks of age. Concurrent infections are common. Winter dysentery in older cattle is another disease presentation.
Diagnosis is supported by history, clinical signs, pathology and supportive tests
Test Package | Animal Age Category | Package Targets |
---|---|---|
Diarrhoea Bovine Package 1 | Neonates (< 4 days of age) | Escherichia coli K99 Salmonella sp. |
Diarrhoea Bovine Package 2 | Pre-weaned calves | Cryptosporidium sp. Salmonella sp. Rotavirus Coronavirus Coccidia |
Diarrhoea Bovine Package 3 | Adults | Salmonella sp. Yersinia sp. Coccidia Round worms |
See below for pricing, sample requirements and other test information.
Click on + in the tables below for sampling requirements and additional test information
Code | Name | Samples required | Species | Turnaround time | Price (excl. GST) | Notes | Test days | |
---|---|---|---|---|---|---|---|---|
DIAB_PK1_1 | Diarrhoea Bovine Package 1 (neonates) - Escherichia coli K99, Salmonella sp. - 1 sample | Faeces
|
Up to 5D |
$98.80 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
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DIAB_PK1_2 | Diarrhoea Bovine Package 1 (neonates) - Escherichia coli K99, Salmonella sp. - 2+ samples (Price per sample) | Faeces
|
Up to 5D |
$83.50 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
|||
DIAB_PK2_1 | Diarrhoea Bovine Package 2 (pre-weaned calves) - Salmonella sp., Cryptosporidium sp., Rotavirus, Coronavirus, Coccidia - 1 sample | Faeces
|
Up to 5D |
$150.85 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
|||
DIAB_PK2_2 | Diarrhoea Bovine Package 2 (pre-weaned calves) - Salmonella sp., Cryptosporidium sp., Rotavirus, Coronavirus, Coccidia - 2+ samples (Price per sample) | Faeces
|
Up to 5D |
$135.00 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
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DIAB_PK3_1 | Diarrhoea Bovine Package 3 (adults) - Salmonella sp., Yersinia sp., Coccidia, round worms - 1 sample | Faeces
|
Up to 5D |
$124.00 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
|||
DIAB_PK3_2 | Diarrhoea Bovine Package 3 (adults) - Salmonella sp., Yersinia sp., Coccidia, round worms - 2+ samples (Price per sample) | Faeces
|
Up to 5D |
$111.00 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Click on + in the tables below for sampling requirements and additional test information
Code | Name | Samples required | Species | Turnaround time | Price (excl. GST) | Notes | Test days | |
---|---|---|---|---|---|---|---|---|
CORONA_PCR | Bovine Coronavirus Real Time PCR* | Faeces, fresh tissue, Swab (PBGS) |
2-3D |
$101.25 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
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CE_K99 | Diarrhoea Bovine Calf Enteric Test Strip – E. coli K99 | Faeces |
1-2D |
$36.25 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
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Various | Faecal Egg Count (Worm Test) | Faeces:
|
1-2D |
$18.90 |
|
Monday- Friday |
||
Various | Faecal Egg Count (2 pools of up to 10 samples) (Worm test) | Faeces:
|
1-2D |
$41.55 |
|
Monday- Friday |
||
Various | Faecal Egg Count (Up to 10 individual samples) (Worm test) | Faeces:
|
1-2D |
$67.20 |
|
Monday- Friday |
||
SIMP_FLOT | Flotation concentration (alternative to wet prep) | Faeces Gastro-intestinal content
|
All species |
1-2D |
$16.85 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday - Friday |
|
FLUKE_QUAL | Fluke (Liver/Stomach) Detection- Individual testing (Not export) (presence/absence) | Faeces:
|
3-5D |
$37.10 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
FLU_QUAL_2 | Fluke (Liver/Stomach) Detection- 2 pools of 5 samples (presence/absence) | Faeces:
|
3-5D |
$58.50 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
FLU_QUAL_5 | Fluke (Liver/Stomach) Detection- 5 pools of 2 samples (presence/absence) | Faeces:
|
3-5D |
$100.65 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
FEC_FLUKE | Fluke Egg Count (Liver/Stomach)- Individual testing- (Not export) | Faeces:
|
3-5D |
$48.80 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
WT_FLUKE_2 | Fluke Egg Count (Liver/Stomach)- 2 pools of 5 samples | Faeces:
|
3-5D |
$96.90 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
WT_FLUKE_5 | Fluke Egg Count (Liver/Stomach)- 5 pools of 2 samples | Faeces:
|
3-5D |
$172.75 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday- Friday |
||
HIST_HE1 | Histopathology – Preparation & Examination | Fixed Tissues- General
Fixed Tissues- Nervous tissues
Fresh Tissues
|
All species |
Up to 10D |
$78.65 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday - Friday |
|
JD_CLT_LIQ | Johne's Disease Individual Faecal & Tissue Culture plus Indicator* | Faeces or fresh tissue Faeces:
Fresh Tissue:
|
14-16W |
$116.60 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. JD Culture requires several consecutive processes; therefore, new cultures are not set up over the Christmas/ New Year period. Samples received during this time will be held at -80°C for processing in the New Year and will have an extended turnaround time. General requirements
|
Sample processing begins on Monday for faeces or Tuesday for tissues |
||
Various | Johne's Disease ELISA | Clotted blood (Red top)
Lith hep (Green top)
Milk |
Up to 5D |
$14.65 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Monday to Friday |
||
PEST_SK_SE | Pestivirus Antigen ELISA (PACE) – Serum or Skin (PRICE TIER A: 1–9 samples) | CATTLEClotted blood (Red top)
Ear Notch- - Dead animals and foetuses
Fresh Tissues- - Dead animals and foetuses
SHEEPClotted blood (red top tube)
Fresh Tissue- Dead animals and foetuses
|
5-7D |
$18.55 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. 3 EDTA and ear notch are not suitable samples for Ovine or Caprine - clotted blood only. |
Batch tested 2-3 times weekly |
||
PEST_SK_SE | Pestivirus Antigen ELISA (PACE) – Serum or Skin (PRICE TIER B:10-19 samples) | CATTLEClotted blood (Red top)
Ear Notch- - Dead animals and foetuses
Fresh Tissues- - Dead animals and foetuses
SHEEPClotted blood (red top tube)
Fresh Tissue- Dead animals and foetuses
|
5-7D |
$13.65 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. 3 EDTA and ear notch are not suitable samples for Ovine or Caprine - clotted blood only. |
Batch tested 2-3 times weekly |
||
PEST_SK_SE | Pestivirus Antigen ELISA (PACE) – Serum or Skin (PRICE TIER C:>20 samples) | CATTLEClotted blood (Red top)
Ear Notch- - Dead animals and foetuses
Fresh Tissues- - Dead animals and foetuses
SHEEPClotted blood (red top tube)
Fresh Tissue- Dead animals and foetuses
|
5-7D |
$11.65 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. 3 EDTA and ear notch are not suitable samples for Ovine or Caprine - clotted blood only. |
Batch tested 2-3 times weekly |
||
PEST_RTPCR | Pestivirus Real–time PCR | CATTLE & SHEEPBlood (Red top)
EDTA blood (Purple top)
Semen
Fresh Tissue- Dead animals and foetuses
CATTLE ONLYMilk
Swab in PBGS
|
2-3D |
$101.25 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
According to demand |
||
ROTA_PCR | Rotavirus Real–time PCR* | Faeces |
2-3D |
$101.25 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
|||
SALM_CLT | Salmonella – Selective culture | Fresh tissue
Faeces
Environmental samples |
All species |
3-7D1 |
$76.00 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. 14 - 7 working days for export, health testing or environmental samples |
Monday to Saturday1 |
|
YERS_CLT | Yersinia sp. culture | Tissue, PM sample, swab |
3-4D |
$42.30 |
D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
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ZOET_MULTI | Diarrhoea Bovine Calf Multi-pathogen Test Strip (E. coli K99, Cryptosporidium sp., Rotavirus, Coronavirus)* | Faeces
|
2D |
$71.80 |
* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. |
Upon request |
Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.
NSW DPIRD's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.
CONTACT DETAILS | |
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Phone | 1800 675 623 |
Operating Hours | 8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only) |
Postal Address | Private Bag 4008, Narellan NSW 2567 |
Veterinary Specimen Advice Form
Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.
For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.
For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.
Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.
Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).
Sample Type | Laboratory | Street Address |
---|---|---|
Animal (including aquatic animals) | NSW Animal and Plant Health Laboratories (APHL) | EMAI, Woodbridge Road, Menangle NSW 2568 |